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[Expression of aquaporin-1 in a guinea pig model of labyrinth destruction]
1Institute of Otolaryngology, General Hospital of PLA, Beijing 100853, China. hxllq@126.com
Objective:
To study the expression of aquaporin-1(AQP-1) in the cochlea and endolymphatic sac in guinea pigs with labyrinth destruction.
Methods:
Chloroform was injected into the tympanum to establish the animal model of labyrinth destruction in guinea pigs, and two-step immunohistochemical method was used to examine the expression of AQP-1 in the cochlea and endolymphatic sac at different time points.
Results:
AQP-1 expression was fluctuant in accordance with the morphological changes of the spiral ligament fibrocytes in the cochlea: destruction of the spiral ligament cells was followed by AQP-1 expression down-regulation, and AQP-1 up-regulation occurred with the cell regeneration. But no such changes were observed in the endolymphatic sac.
Conclusion:
AQP-1 may take part in the maintenance of the structural stability of the spiral ligament.
