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Related Experiment Videos

Calbindin-D28K dynamically controls TRPV5-mediated Ca2+ transport.

Tim T Lambers1, Frank Mahieu, Elena Oancea

  • 1Department of Physiology, Radboud University Nijmegen Medical Centre, The Netherlands.

The EMBO Journal
|June 10, 2006
PubMed
Summary
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Calbindin-D(28K) (CaBP(28K)) dynamically buffers cytosolic calcium near the TRPV5 channel in kidney cells. This association regulates calcium transport and prevents channel inactivation, enhancing overall calcium absorption.

Area of Science:

  • Cellular Physiology
  • Epithelial Transport
  • Calcium Homeostasis

Background:

  • Calbindin-D(28K) (CaBP(28K)) facilitates calcium diffusion and buffers cytosolic calcium in Ca(2+)-transporting epithelia.
  • CaBP(28K) is known to colocalize with the epithelial calcium channel TRPV5 in renal cells.

Purpose of the Study:

  • To investigate the direct interaction between CaBP(28K) and TRPV5.
  • To elucidate the functional consequences of CaBP(28K) association with TRPV5 on calcium transport.

Main Methods:

  • Protein-binding analysis
  • Subcellular fractionation
  • Evanescent-field microscopy
  • (45)Ca(2+) uptake measurements
  • Electrophysiological recordings

Related Experiment Videos

  • Transcellular calcium transport assays

    • Main Results:

    • CaBP(28K) translocates to the plasma membrane and directly binds to TRPV5 at low cytosolic calcium levels.
    • Associated CaBP(28K) buffers calcium flux through TRPV5, preventing channel inactivation.
    • This interaction facilitates higher transcellular calcium transport rates.

    Conclusions:

    • CaBP(28K) acts as a dynamic calcium buffer in proximity to the TRPV5 channel.
    • Direct association of CaBP(28K) with TRPV5 is crucial for regulating calcium influx and transport in renal epithelia.