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Circulating ICAM-1 isoforms: diagnostic prospects for inflammatory and immune disorders.
R Seth1, F D Raymond, M W Makgoba
1Department of Chemical Pathology, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK.
Lancet (London, England)
|July 13, 1991
Summary
Researchers detected free circulating Intercellular Adhesion Molecule-1 (ICAM-1, CD54) in human serum using a sensitive assay. This finding opens new avenues for diagnosing and monitoring immune and inflammatory disorders.
Area of Science:
- Immunology
- Biochemistry
- Medical Diagnostics
Background:
- Intercellular Adhesion Molecule-1 (ICAM-1, CD54) is a key marker of immune activation.
- Previously, evidence relied on tissue staining as free circulating ICAM-1 was undetected.
Purpose of the Study:
- To detect and characterize free circulating ICAM-1 in human serum.
- To explore the potential diagnostic and therapeutic implications of circulating ICAM-1.
Main Methods:
- Utilized monoclonal antibodies against ICAM-1.
- Employed a sensitive chemiluminescence technique for detection.
- Applied non-denaturing gel electrophoresis for isoform analysis.
Main Results:
- Successfully detected free circulating ICAM-1 in serum from healthy volunteers.
- Observed significant variation in ICAM-1 concentrations among individuals.
- Identified at least three circulating ICAM-1 isoforms with varying proportions.
Conclusions:
- Circulating ICAM-1 can be detected and quantified in human serum.
- The presence and isoforms of ICAM-1 may serve as valuable biomarkers.
- Findings support ICAM-1's role in investigating inflammatory, neoplastic, and immune disorders.
