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Updated: Aug 7, 2026

Receptor Autoradiography Protocol for the Localized Visualization of Angiotensin II Receptors
Published on: June 7, 2016
Specific receptor for angiotensinogen on human renal cells
Nan Pan1, Jiangming Luo, Steven J Kaiser
1Marshfield Clinic Research Foundation, 1000 North Oak Avenue, Marshfield, WI 54449, USA.
Researchers found an angiotensinogen (AGT) receptor on human kidney cells. This discovery suggests the AGT receptor is a key part of the kidney's renin-angiotensin system (RAS).
Area of Science:
- Nephrology
- Endocrinology
- Molecular Biology
Background:
- Previous research identified an angiotensinogen (AGT) receptor on placental cells.
- The human kidney possesses a tissue-specific renin-angiotensin system (RAS).
Purpose of the Study:
- To investigate the presence of an AGT receptor on human renal proximal tubule epithelial cells.
Main Methods:
- Human renal proximal tubule epithelial cells were cultured.
- Binding assays utilized iodinated angiotensinogen ((125)I-AGT) with and without unlabeled AGT.
- Radioactivity measurement quantified AGT binding and internalization.
Main Results:
- Human renal proximal tubule epithelial cells exhibited time-dependent binding of (125)I-AGT.
- Binding was specifically inhibited by unlabeled AGT, indicating receptor interaction.
- Scatchard analysis revealed a single class of high-affinity AGT binding sites (Kd=1.73 nmol, Bmax=23.39 pmol/10(6) cells).
- 70% of bound AGT was internalized by the cells.
Conclusions:
- Evidence supports the presence of an AGT receptor on human renal proximal tubule epithelial cells.
- The identified AGT receptor is likely an integral component of the renal RAS.
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