A microfluidic fluorous solid-phase extraction chip for purification of amino acids
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Published on: October 15, 2013
Guoqing Hu1, Jacky S H Lee, Dongqing Li
1Department of Mechanical Engineering, Vanderbilt University, VU Station B 351592, 2301 Vanderbilt Place, Nashville, TN 37235-1592, USA.
Abstract:
An electrokinetically-driven microfluidic chip was developed to realize beads-based solid-phase extraction (SPE) of amino acids. This chip uses a two-level (deep/shallow) poly(dimethylsiloxane) (PDMS) microchannel network to confine the fluorous reversed-phase silica beads within the SPE chamber. The mixture of fluorous tagged and non-tagged amino acids was carried into the fluorous solid-phase extraction (F-SPE) chamber by electrokinetic pumping and was successfully separated and extracted. By adding a reference material to the sample, the extraction efficiency of the eluted fluorous-tagged amino acid was calculated using the detection results from mass spectrometry (MS). The F-SPE microchips showed good reproducibility and efficiency, yielding an average extraction efficiency of 55% with a RSD of 10.6% under the typical experimental conditions.
