Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Expression of LASS2 controlled by LAG1 or ADH1 promoters cannot functionally complement Lag1p.

Yao Yu1, Hong Lu, Hui Pan

  • 1State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, PR China.

Microbiological Research
|June 13, 2006
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Molecular mechanisms of osteoarthritis using gene microarrays.

Acta histochemica·2014
Same author

An overview of hedgehog signaling in fibrosis.

Molecular pharmacology·2014
Same author

Enhanced utility of family-centered diagnostic exome sequencing with inheritance model-based analysis: results from 500 unselected families with undiagnosed genetic conditions.

Genetics in medicine : official journal of the American College of Medical Genetics·2014
Same author

Therapeutic imaging window of cerebral infarction revealed by multisequence magnetic resonance imaging: An animal and clinical study.

Neural regeneration research·2014
Same author

Microwave synthesis of microstructured and nanostructured metal chalcogenides from elemental precursors in phosphonium ionic liquids.

Journal of the American Chemical Society·2014
Same author

Cr(VI) reduction and Cr(III) immobilization by Acinetobacter sp. HK-1 with the assistance of a novel quinone/graphene oxide composite.

Environmental science & technology·2014

Human LASS2 and its fragment LASS2DeltaHOX cannot functionally complement yeast Lag1p. These findings indicate that the human homologues do not rescue the slow growth defect in yeast ceramide synthase mutants.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Yeast Genetics

Background:

  • The LAG1 gene in Saccharomyces cerevisiae encodes a protein crucial for ceramide synthase activity and substrate specificity.
  • Disruption of LAG1 and its homologue LAC1 leads to a slow growth phenotype in yeast under specific genetic conditions.
  • LASS2 is identified as a human homologue of Lag1p, possessing conserved domains like TLC and HOX.

Purpose of the Study:

  • To investigate the functional complementation of yeast Lag1p by its human homologue LASS2 and a truncated version (LASS2DeltaHOX).
  • To determine if LASS2 or LASS2DeltaHOX can rescue the slow growth defect in yeast double mutants lacking LAG1 and LAC1.

Main Methods:

  • Functional complementation assays were performed using shuffling tests and tetrad analyses in Saccharomyces cerevisiae.

Related Experiment Videos

  • The ability of LASS2 and LASS2DeltaHOX to rescue the yeast slow growth phenotype was assessed under the control of both the native LAG1 promoter and the strong ADH1 promoter.

    • Main Results:

    • Neither full-length LASS2 nor the LASS2DeltaHOX fragment could rescue the slow growth defect of the yeast lag1/lac1 double mutant.
    • Complementation was unsuccessful regardless of whether the genes were expressed from the endogenous LAG1 promoter or the strong ADH1 promoter.

    Conclusions:

    • Human LASS2 and its HOX domain-lacking fragment LASS2DeltaHOX do not possess functional complementation activity for yeast Lag1p.
    • These results suggest a lack of functional conservation or essential domain requirements for Lag1p activity between yeast and humans.