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Electroporation of Mycobacteria
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Electroporation of Mycobacteria

Published on: May 23, 2008

Efficient transformation of Legionella pneumophila by high-voltage electroporation

Ding-Qiang Chen1, Shao-Song Huang, Yong-Jun Lu

  • 1Department of Biochemistry and State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-sen Zhongshan University, Guangzhou, China.

Microbiological Research
|June 13, 2006
PubMed

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Transformation01:26

Transformation

Microbial communities are dynamic environments where cell lysis releases free DNA into the surroundings. Other cells can take up this extracellular DNA through a process known as transformation.When a cell incorporates this foreign DNA into its genome, resulting in genetic modification, the process is known as transformation. Cells capable of this process are termed competent. Competence can be natural, as observed in certain bacteria and archaea, or artificially induced in the...