High-precision DNA microsatellite genotyping in Duchenne muscular dystrophy families using ion-pair reversed-phase
Nasser Attia Elhawary1, Rabah Mohamed Shawky, Nermine Elsayed
1Department of Molecular Genetics, Medical Genetics Center, Egypt. elhawaryna@gmail.com
Objectives:
Genetic typing of the intronic (CA)n loci within the dystrophin gene was exploited for linkage analysis and diagnosis of carrier status.
Design And Methods:
IP-RP-HPLC was developed for genotyping of 98 chromosomes.
Results:
The typing of 2-bp STRs with mean SD of +/- 0.43 bp ensured an allele's identity with 99.7% confidence.
Conclusion:
The automation enabled the accurate sizing of unlabeled alleles with an excellent reproducibility of 100% with significant labor saving and reduced reagent costs.
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