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Attaching Biological Probes to Silica Optical Biosensors Using Silane Coupling Agents
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Synthesis of biotinylated photoaffinity probes based on arylsulfonamide gamma-secretase inhibitors

Haruhiko Fuwa1, Kenichi Hiromoto, Yasuko Takahashi

  • 1Graduate School of Pharmaceutical Sciences, University of Tokyo, Bunkyo-ku, Japan. hfuwa@bios.tohoku.ac.jp

Bioorganic & Medicinal Chemistry Letters
|June 13, 2006
PubMed

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Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...