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The Cultivation, Growth, and Viability of Lactic Acid Bacteria: A Quality Control Perspective
Published on: June 16, 2022
[Isolation of an amylolytic lactic acid bacterium and its application on lactic acid production from kitchen waste]
Xu-ming Wang1, Qun-hui Wang, Nan-qi Ren
1School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China.
Abstract:
In order to realize resource recycling of kitchen waste, the enhancement of fermentative production of lactic acid (LA) by a strain of amylolytic lactic acid bacterium (ALAB) isolated from kitchen waste was investigated. A total of 6 strains of ALAB were isolated from anaerobically fermented kitchen waste. Among these isolates, the strain FH164 exhibited the highest starch-hydrolyzing rate and LA production. 32.67 g x L(-1) of LA was produced from 40.50 g x L(-1) of soluble starch by strain FH164 at pH 5.5-6.0 in 48 h of fermentation. On the basis of its morphological and physio-biochemical characteristics as well as 16S rDNA sequence, the strain FH164 was tentatively identified as Streptococcus sp.. 28.23 g x L(-1) of LA was obtained from kitchen waste by the strain FHl64 employing an open fermentation mode (substrate non-autoclaved), which was 19.2% higher than that of the fermentation without inoculum (control).
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