Related Experiment Video
Updated: Aug 7, 2026
06:04
Study Motor Skill Learning by Single-pellet Reaching Tasks in Mice
Published on: March 4, 2014
Differential role for CBP and p300 CREB-binding domain in motor skill learning
Ana M M Oliveira1, Ted Abel, Paul K Brindle
1Department of Biology, University of Pennsylvania, Philadelphia, PA, USA.
Behavioral Neuroscience
|June 14, 2006
Summary
Cyclic adenosine monophosphate response element binding protein (CREB) binding protein (CBP) and its homolog p300 play distinct roles in motor learning. The CREB-CBP interaction is crucial for motor skill acquisition, unlike the CREB-p300 interaction.
Area of Science:
- Neuroscience
- Molecular Biology
- Genetics
Background:
- Cyclic adenosine monophosphate response element binding protein (CREB) binding protein (CBP) and E1A binding protein (p300) are homologous transcriptional coactivators.
- While CBP and p300 have distinct roles in embryogenesis and hematopoiesis, their functions in the nervous system are less understood.
Purpose of the Study:
- To investigate the differential roles of CBP and p300 in motor skill learning.
- To determine the specific contribution of the CREB-CBP and CREB-p300 interactions to motor learning.
Main Methods:
- Utilized mutant mice with specific KIX domain mutations in CBP and p300.
- Assessed motor learning capabilities in these mutant mice.
- Examined CREB knock-out mice for comparison.
Main Results:
- Mice with a mutated CREB-binding (KIX) domain in CBP showed significant motor learning deficits.
- Mice with an analogous mutation in p300's KIX domain exhibited normal motor learning.
- CREB knock-out mice displayed motor learning deficits comparable to CBP-KIX mutant mice.
Conclusions:
- CBP and p300 play genetically distinct roles in motor skill learning.
- The interaction between CREB and CBP is critical for motor learning, suggesting it is more limiting than the CREB-p300 interaction.
Related Concept Videos
02:23Master Transcription Regulators
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
01:14Role of Cerebellum and Prefrontal Cortex in Memory
The cerebellum, while traditionally associated with motor control, also plays a crucial role in memory, particularly in procedural memory, which involves learning motor tasks that become automatic through repetition. For example, studies have shown that when the cerebellum is damaged, individuals or animals lose the ability to learn conditioned motor responses, such as the conditioned eye-blink response in classical conditioning experiments with rabbits. This study demonstrates the cerebellum's...
01:16Calmodulin-dependent Signaling
Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...