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A cell line (LIM 2463) derived from a tubulovillous adenoma of the rectum
R H Whitehead1, P van Eeden, R E Lukeis
1Ludwig Institute for Cancer Research, Melbourne Tumour Biology Branch, Royal Melbourne Hospital, Victoria, Australia.
Abstract:
We describe a new epithelial cell line (LIM 2463) derived from tubulovillous adenoma of the rectum. The cells grow as organoids and secrete large amounts of mucus. The cells are polarized, with a microvillar brush border, and express dipeptidyl peptidase IV in a polarized manner. No staining was seen with 3 other antibodies directed against other brush-border hydrolases or disaccharidases. Focal polarized staining was obtained with 2 antibodies directed against other brush-border-associated peptides. The cells are aneuploid with a distinctive karyotype (48, XX, +9, +13). All attempts to clone the cells in semi-solid agar or to grow them as xenografts in nude athymic mice have failed.