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Published on: November 10, 2017
Concordant association of lipid gene variation with a combined HDL/LDL-cholesterol phenotype in two European
Anja Bauerfeind1, Hans Knoblauch, Michael C Costanza
1Department of Bioinformatics, Max Delbruck Center for Molecular Medicine, Berlin, Germany.
Objective:
SNP/phenotype associations are difficult to validate. This comparative study demonstrates significant contribution of candidate genes to the variation of a complex cholesterol phenotype, measured in two general populations by a gene-based approach.
Methods:
Independent samples of normolipidemic subjects from two Caucasian populations (371 Swiss and 157 Germans) were selected for a case-control-study (high LDL/low HDL versus low LDL/high HDL) with SNP genotypes as independent factors. We examined locus-specific common SNPs that densely cover the genomic regions of 10 lipid genes.
Results:
Genotype effects were concordant in both ethnic samples, showing that APOE, ABCA1, CETP, and to a lesser degree LDLR, LIPC, and PLTP explained a substantial part of the genetic variation, whereas LPL was associated in only one sample. APOA1, LCAT, and SRB1 exerted no measurable influence.
Conclusion:
This comparison showed that sets of common SNPs representing candidate regions reproducibly validate significant linkage disequilibrium association with a complex metabolic trait.
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