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Understanding microbial metabolism.

Diana M Downs1

  • 1Department of Bacteriology, College of Agriculture and Life Sciences, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA. downs@bact.wisc.edu

Annual Review of Microbiology
|June 15, 2006
PubMed
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Understanding microbial metabolism requires integrating computational (in silico), laboratory (in vitro), and live organism (in vivo) methods. This systems-level approach moves beyond studying individual parts to modeling the entire metabolic network for broad biological applications.

Area of Science:

  • Biochemistry
  • Microbiology
  • Systems Biology

Background:

  • Metabolism is fundamental to all life, with extensive research on microbial metabolic components and regulation.
  • Current technologies enable a shift from studying metabolic parts to understanding microbial metabolism as a system.

Purpose of the Study:

  • To review approaches for gaining insight into microbial metabolism.
  • To emphasize the necessity of combining diverse methodologies for a comprehensive understanding.

Main Methods:

  • Defining metabolic components through rigorous characterization.
  • Discovering novel components and connections using in vivo-driven approaches.
  • Integrating in silico, in vitro, and in vivo methodologies.

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Main Results:

  • A systems-level understanding of microbial metabolism is achievable.
  • Detailed knowledge of components and connections facilitates mathematical modeling.
  • Combined approaches yield deeper insights than isolated methods.

Conclusions:

  • A comprehensive understanding of microbial metabolism necessitates the synergistic application of in silico, in vitro, and in vivo methods.
  • This systems-level approach to microbial metabolism has implications across all biological disciplines.