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Susceptibility of yeast isolates from cattle with otitis to aqueous solution of povidone iodine and to alcohol-ether

E R Duarte1, J S Hamdan

  • 1Department of Microbiology, Biological Sciences Institute, Federal University of Minas Gerais, Brazil.

Medical Mycology
|June 15, 2006
PubMed

Insights

Povidone iodine and alcohol-ether solutions show in vitro efficacy against yeast causing bovine otitis. Further clinical evaluation is needed for topical treatment and control strategies.

Area of Science:

  • Veterinary Mycology
  • Antimicrobial Susceptibility Testing
  • Bovine Otitis Therapeutics

Background:

  • Lipid-dependent Malassezia species, Candida spp., and Rhodotorula mucilaginosa are implicated in bovine parasitic otitis.
  • Effective topical treatments are crucial for managing bovine otitis, yet susceptibility data for common antiseptics is limited.
  • Understanding yeast susceptibility is key to developing targeted therapeutic interventions for otitis externa in cattle.

Purpose of the Study:

  • To evaluate the in vitro susceptibility of yeast isolates from cattle with otitis to povidone iodine and alcohol-ether solutions.
  • To determine the microbicidal effect (ME) and minimum inhibitory concentration (MIC) of these antiseptics against relevant yeast species.
  • To assess the potential of these antiseptics as topical treatments for bovine otitis.

Main Methods:

  • Sixty-three yeast isolates from cattle with otitis were tested.
  • Antiseptic effectiveness was assessed using the European suspension test (povidone iodine 1% and 0.5% v/v; alcohol-ether 1:1 v/v).
  • Minimum inhibitory concentration (MIC) was determined using the urea broth macrodilution method.

Main Results:

  • Both povidone iodine and alcohol-ether solutions demonstrated microbicidal effect (ME > 5) against most yeast strains within 1-5 minutes.
  • Exceptions included the alcohol-ether solution against two Candida tropicalis strains.
  • Malassezia sympodialis and Rhodotorula mucilaginosa were more susceptible to povidone iodine, while Malassezia furfur was less susceptible. Malassezia sympodialis showed higher susceptibility to the alcohol-ether solution.

Conclusions:

  • Povidone iodine and alcohol-ether solutions exhibit significant in vitro efficacy against key yeast pathogens associated with bovine otitis.
  • Variations in susceptibility were observed among different yeast species and between the two antiseptic agents.
  • Clinical evaluation of these antiseptics for topical treatment and control of bovine otitis is warranted, considering their impact on ear microbiota.

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