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Technical note: a rapid pulsed-field gel electrophoresis method for analysis of bifidobacteria

E P Briczinski1, R F Roberts

  • 1Department of Food Science, The Pennsylvania State University, University Park 16802, USA.

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Two-dimensional Gel Electrophoresis

Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as  cells...