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Related Experiment Videos

Tissue-engineered axially vascularized contractile skeletal muscle.

Gregory H Borschel1, Douglas E Dow, Robert G Dennis

  • 1Section of Plastic Surgery and Department of Biomedical Engineering, University of Michigan, Ann Arbor, USA.

Plastic and Reconstructive Surgery
|June 15, 2006
PubMed
Summary

Engineered skeletal muscle constructs were successfully grown in vivo with integrated vascular networks. These three-dimensional tissues exhibit contractile force and histological characteristics comparable to native muscle.

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Area of Science:

  • Biomedical Engineering
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Scaling tissue-engineered muscle is limited by vascularization challenges.
  • This study introduces a method to overcome size limitations using an axial vascular pedicle.

Purpose of the Study:

  • To engineer three-dimensional contractile skeletal muscle in vivo.
  • To create vascularized muscle constructs with functional properties.

Main Methods:

  • Rat myoblasts were cultured and suspended in a fibrinogen hydrogel within silicone chambers.
  • Chambers were implanted around the femoral vessels of recipient rats for 3 weeks.
  • Constructs were explanted for functional and histological analysis.

Main Results:

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  • Engineered muscle constructs generated longitudinal contractile force upon electrical stimulation.
  • Histology confirmed myoblast fusion into multinucleated myotubes and extensive capillary ingrowth.
  • Functional properties mirrored those of developing native skeletal muscle.

Conclusions:

  • In vivo engineering of three-dimensional, vascularized skeletal muscle is feasible.
  • The resulting engineered muscle tissues possess native-like histological and functional characteristics.