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Updated: Aug 7, 2026

Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis
Published on: February 18, 2020
Periodic pulses of calcium ions in a chemical system
Krisztina Kurin-Csörgei1, Irving R Epstein, Miklós Orban
1Department of Inorganic and Analytical Chemistry, L. Eötvös University, H-1518 Budapest 112, P.O. Box 32, Hungary.
Abstract:
By coupling the bromate-sulfite-ferrocyanide oscillating chemical reaction with the complexation of calcium ion by EDTA, we construct a system that generates periodic pulses of free Ca(2+) with an amplitude of 2 orders of magnitude and a period of ca. 20 min. These pulses may be observed either with a calcium ion-selective electrode or with Arsenazo(III) as an indicator. We describe the systematic design procedure and the properties of this first abiotic calcium-based chemical oscillator.
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