Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Automated microscopy system for mosaic acquisition and processing.

S K Chow1, H Hakozaki, D L Price

  • 1National Center for Microscopy and Imaging Research, University of California San Diego, 9500 Gilman Drive, Basic Science Building, Room 1000, La Jolla, CA 92093-0608, U.S.A.

Journal of Microscopy
|June 16, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

30-day morbidity and mortality after transoral robotic surgery for human papillomavirus (HPV) associated oropharyngeal squamous cell carcinoma: A retrospective analysis of two prospective adjuvant de-escalation trials (MC1273 & MC1675).

Oral oncology·2023
Same author

Non-opioid analgesics and post-operative pain following transoral robotic surgery for oropharyngeal cancer.

The Journal of laryngology and otology·2022
Same author

Prototype of Kepler Processing Workflows For Microscopy And Neuroinformatics.

Procedia computer science·2017
Same author

Structure of molten yttrium aluminates: a neutron diffraction study.

Journal of physics. Condensed matter : an Institute of Physics journal·2017
Same author

Magnetic critical scattering in solid Co<sub>80</sub>Pd<sub>20</sub>.

Journal of physics. Condensed matter : an Institute of Physics journal·2017
Same author

Baseline characteristics of the patient population in the Saxagliptin Assessment of Vascular Outcomes Recorded in patients with diabetes mellitus (SAVOR)-TIMI 53 trial.

Diabetes/metabolism research and reviews·2013
Same journal

In operando imaging of the space-charge region in a 4H-SiC MOSCAP using STEM-EBIC.

Journal of microscopy·2026
Same journal

The future of DXA: How AI is transforming bone health diagnostics.

Journal of microscopy·2026
Same journal

The Origins of Ploem's Filter Cube: A Pandora's Box.

Journal of microscopy·2026
Same journal

The reproducibility gap in graph neural network workflows for cell dynamics: A checklist-driven case study.

Journal of microscopy·2026
Same journal

Assessing the reproducibility of a bioimage analysis workflow characterising tissue flow in Drosophila.

Journal of microscopy·2026
Same journal

Modular training resources for bioimage analysis.

Journal of microscopy·2026
See all related articles

This study introduces an automated system for creating large, high-resolution images of biological tissues using multiphoton microscopy. The system enables detailed, wide-field views of specimens by stitching together thousands of individual images.

Area of Science:

  • Microscopy
  • Biotechnology
  • Neuroscience

Background:

  • High-resolution imaging of large biological specimens is challenging.
  • Existing methods often sacrifice resolution for field of view or vice versa.
  • Mosaic imaging offers a solution by combining multiple high-resolution images.

Purpose of the Study:

  • To develop and describe an automated system for mosaic acquisition and processing.
  • To enable high-resolution imaging of large biological specimens using multiphoton microscopy.
  • To address the challenges of creating very large-scale mosaics from complex biological samples.

Main Methods:

  • Utilized a fast-scanning, multiphoton, confocal microscope.
  • Integrated a motorized, high-precision stage.

Related Experiment Videos

  • Developed custom software for automatic image acquisition, normalization, alignment, and stitching.
  • Applied the system to brain tissue labeled with multiple fluorescent probes.

    • Main Results:

    • Successfully created a system for automatic mosaic acquisition and processing.
    • Demonstrated capability to acquire datasets of thousands to tens of thousands of images per mosaic.
    • Enabled routine creation of very large-scale mosaics at near light microscopy resolution limits.
    • Provided detailed, contextualized views of biological specimens.

    Conclusions:

    • The automated system effectively overcomes challenges in large-scale mosaic creation.
    • This technology facilitates high-resolution, wide-field imaging of biological specimens.
    • The system is crucial for detailed analysis of complex tissues like brain samples.