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Updated: Aug 7, 2026

Facile Preparation of 4-Substituted Quinazoline Derivatives
Published on: February 15, 2016
Chiral separation of four fluoroquinolone compounds using capillary electrophoresis with
Shanshan Zhou1, Jin Ouyang, Willy R G Baeyens
1Department of Chemistry, Beijing Normal University, Beijing 100875, China.
Abstract:
A capillary zone electrophoresis (CZE) investigation on the enantiomeric separation of lomefloxacin, gatifloxacin, pazufloxacin and ofloxacin was undertaken. Resolution of the enantiomers was achieved using hydroxypropyl-beta-cyclodextrin (HP-beta-CD) as the chiral selector. Parameters influencing separation include cyclodextrin concentration, separational potential, pH and organic additive are discussed. A buffer consisting of 70 mM phosphate and 40 mM HP-beta-CD at pH 3.96 was found to be highly efficient for the separation of lomefloxacin, at pH 3.90 for gatifloxacin, at pH 5.04 for pazufloxacin and at pH 2.16 for ofloxacin. To the best of our knowledge, this is the first report on the enantiomeric resolution of lomefloxacin and gatifloxacin applying CE.
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