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Related Experiment Videos

Applications of restriction fragment length polymorphism.

S Narayanan1

  • 1Department of Pathology, New York Medical College/Metropolitan Hospital Center NY 10029.

Annals of Clinical and Laboratory Science
|July 1, 1991
PubMed
Summary

Restriction fragment length polymorphism (RFLP) identifies DNA variations using restriction enzymes. These variations, stemming from variable number tandem repeats (VNTRs), are key for DNA fingerprinting, paternity tests, and as genetic disease markers.

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Area of Science:

  • Molecular Biology
  • Genetics

Background:

  • Restriction endonuclease enzymes enable the identification of specific DNA cleavage sites.
  • Polymorphic regions in DNA fragments can be detected using these enzymes.

Purpose of the Study:

  • To review materials and methodologies for detecting Restriction Fragment Length Polymorphism (RFLP).
  • To highlight the application of RFLP in DNA fingerprinting, paternity testing, and as genetic disease markers.
  • To emphasize the role of amplification procedures, specifically the polymerase chain reaction (PCR), in RFLP detection.

Main Methods:

  • Cleavage of deoxyribonucleic acid (DNA) using restriction endonucleases.
  • Analysis of DNA fragments to identify variations in the number of tandem repeats (VNTRs).
  • Application of polymerase chain reaction (PCR) for amplification of DNA segments.

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Main Results:

  • Restriction fragment length polymorphism (RFLP) arises from variations in VNTRs.
  • VNTR sequences provide unique individual identification, crucial for forensic and paternity applications.
  • RFLP can serve as a genetic disease marker when located near a disease gene.

Conclusions:

  • RFLP analysis is a powerful tool for individual identification and genetic analysis.
  • The utility of RFLP as a genetic marker depends on its proximity to the disease gene.
  • Polymerase chain reaction (PCR) enhances the detection and application of RFLP analysis.