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Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions
Published on: August 2, 2018
TSGA10 prevents nuclear localization of the hypoxia-inducible factor (HIF)-1alpha
Sonja Hägele1, Babak Behnam, Emanuela Borter
1Cell Physiology Group, Martin-Luther University of Halle, Magdeburger Strasse 2, D-06097 Halle, Germany.
Abstract:
The hypoxia-inducible factor (HIF)-1 is a transcriptional regulator of genes involved in oxygen homeostasis. We previously described testis-specific isoforms of HIF-1alpha (mHIF-1alphaI.1 and hHIF-1alphaTe). Using mHIF-1alpha exon I.1 knock-out mice we confirmed the specific expression of mHIF-1alphaI.1 in the sperm tail. A protein-protein interaction between HIF-1alpha and the testis specific gene antigen 10 (TSGA10) was identified by yeast two-hybrid screening. TSGA10 is expressed in testis but also in other organs and malignant tissues. Immunofluorescence analysis indicated that the C-terminal part of TSGA10 accumulates in the midpiece of spermatozoa, where it co-localizes with HIF-1alpha. HIF-1alpha nuclear localization and HIF-1 transcriptional activity were significantly affected by overexpressed TSGA10.
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