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Related Experiment Videos

Sensitive quantification of apomorphine in human plasma using a LC-ESI-MS-MS method.

Emuri Abe1, Jean-Claude Alvarez

  • 1Laboratoire de Pharmacologie-Toxicologie, Centre Hospitalier Universitaire Raymond Poincaré, AP-HP, 104 Boulevard R. Poincaré, 92380 Garches France.

Therapeutic Drug Monitoring
|June 17, 2006
PubMed
Summary

A new liquid chromatography-mass spectrometry method accurately quantifies apomorphine in human plasma using a small sample volume. This technique is ideal for therapeutic drug monitoring and pharmacokinetic studies.

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Area of Science:

  • Analytical Chemistry
  • Pharmacology

Background:

  • Apomorphine is a dopamine agonist used in treating Parkinson's disease and erectile dysfunction.
  • Accurate quantification of apomorphine in biological matrices is crucial for therapeutic drug monitoring and pharmacokinetic studies.

Purpose of the Study:

  • To develop and validate a sensitive and specific analytical method for the identification and quantification of apomorphine in human plasma.

Main Methods:

  • Liquid chromatography coupled with ion trap mass spectrometry (LC-MS) using electrospray ionization.
  • Liquid-liquid extraction of apomorphine from 0.5 mL human plasma with diethyl ether.
  • Separation on a C18 column with an isocratic mobile phase of 2 mM NH4COOH buffer (pH 3.8)/acetonitrile (50/50).
  • Detection and quantification using full-scan tandem mass spectrometry, monitoring specific precursor and daughter ions.

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Main Results:

  • The method demonstrated linearity over a concentration range of 0.025 to 20 ng/mL.
  • Limits of detection and quantification were 0.010 ng/mL and 0.025 ng/mL, respectively.
  • High accuracy (99.5-104.2%) and precision (intraday <10.1%, interday <6.6%) were achieved.
  • Quantification of apomorphine in a patient sample after subcutaneous administration.

Conclusions:

  • The developed LC-MS method provides sensitive, specific, and reliable quantification of apomorphine in human plasma.
  • The method's convenience and low sample volume requirement make it suitable for therapeutic drug monitoring and pharmacokinetic investigations.