Related Experiment Videos
Cloning and sequencing the urocanase gene (hutU) from Pseudomonas putida
M Fessenmaier1, R Frank, J Retey
1Institute of Organic Chemistry, University of Karlsruhe, Germany.
FEBS Letters
|July 29, 1991
Abstract:
A clone harbouring the entire urocanase gene (hutU) was obtained from a genomic library of Pseudomonas putida using oligonucleotide probes synthesised on the basis of known flanking sequences. One subunit of urocanase consists of 556 amino acids and has a molecular mass of 60,771 Da.