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Semiautomated autologous bone marrow processing for transplantation
L Pierelli1, G Menichella, A Paoloni
1Istituto di Semeiotica Medica, Università, Cattolica del Sacro Cuore, Rome, Italy.
Haematologica
|March 1, 1991
Abstract:
This paper describes the development of a semiautomated procedure for autologous bone marrow processing, prior to ex vivo manipulation and/or cryopreservation. This procedure was employed with a pediatric bowl (125 ml Latham bowl) and the automated DuPont Stericell processor. We have obtained a mononuclear cell recovery of 85% and a hemopoietic progenitor cell recovery of 81% (CFU-GM; BFU-E), with a red cell removal of 84%. We believe that a reliable and standardized bone marrow processing procedure is the basic necessity for a bone marrow transplantation program.