Collection, processing and storage of peripheral blood stem cells (PBSC)

Area of Science:

• Hematology
• Oncology
• Stem Cell Transplantation

Background:

• Hematological malignancies pose significant treatment challenges.
• Autologous blood stem cell transplantation (ABSCT) is a critical therapeutic option.
• Effective mobilization of hemopoietic precursor cells is essential for successful ABSCT.

Purpose of the Study:

• To evaluate a method for mobilizing hemopoietic precursor cells into peripheral blood (PB).
• To assess the efficacy of collecting and cryopreserving these cells for ABSCT.
• To analyze the recovery and viability of cryopreserved stem cells post-thaw.

Main Methods:

• Mobilization of hemopoietic precursor cells via chemotherapy-induced myelosuppression and subsequent overshoot.
• Collection of peripheral blood stem cells using continuous flow leukapheresis (Fenwall CS 3000).
• Cryopreservation of mononuclear cell suspension in 10% DMSO in liquid nitrogen.

Main Results:

• A 15-20 fold increase in PB CFU-GM observed 15-20 days post-chemotherapy.
• Successful collection of peripheral blood stem cells during rapid platelet and WBC count recovery.
• Post-thaw CFU-GM recovery ranged from 13.8% to 81.5%, with 55-70% cell viability.

Conclusions:

• Chemotherapy-induced mobilization followed by leukapheresis is effective for collecting stem cells for ABSCT.
• Cryopreservation preserves stem cell viability and function for subsequent transplantation.
• This approach supports the application of ABSCT in patients with hematological malignancies.