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Design considerations for a web-based database system of ELISpot assay in immunological research

Jingming Ma1, Tim Mosmann, Hulin Wu

  • 1Department of Biostatistics and Computational Biology, University of Rochester, NY 14642, USA.

AMIA ... Annual Symposium Proceedings. AMIA Symposium
|June 17, 2006
PubMed

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Enzyme-Linked Immunosorbent Assay

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Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.