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Depolarization reverses age-related decrease of spontaneous transmitter release.

W B Alshuaib1, M A Fahim

  • 1Andrus Gerontology Center, University of Southern California, Los Angeles 90089-0191.

Journal of Applied Physiology (Bethesda, Md. : 1985)
|May 1, 1991
PubMed
Summary
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Aging increases calcium entry into nerve terminals, leading to higher spontaneous transmitter release in older mice. This age-related change affects neuromuscular junctions and neurotransmission.

Area of Science:

  • Neuroscience
  • Aging Research
  • Molecular Biology

Background:

  • Spontaneous transmitter release is crucial for neuromuscular function.
  • Aging alters neurotransmission, but mechanisms at the nerve terminal are not fully understood.

Purpose of the Study:

  • To investigate the impact of aging on spontaneous transmitter release at the soleus nerve terminal.
  • To examine the role of extracellular calcium concentration in age-dependent changes in neurotransmission.

Main Methods:

  • Intracellular recording of miniature end-plate potentials (MEPPs) in soleus nerve terminals.
  • Utilizing varying extracellular potassium and calcium concentrations in Krebs solutions.
  • Comparing young (10 mo) and old (24 mo) C57BL/6J mice.

Related Experiment Videos

Main Results:

  • Resting MEPP frequency was lower in old nerve terminals compared to young ones.
  • Upon depolarization, MEPP frequency was higher in old nerve terminals than in young ones, especially at higher calcium concentrations.
  • Observed periodic oscillations in MEPP frequency, inversely proportional to spontaneous transmitter release.

Conclusions:

  • Depolarization increases nerve terminal membrane permeability to calcium via voltage-dependent channels.
  • Aging enhances voltage-dependent calcium entry at the soleus nerve terminal.
  • These findings highlight age-related alterations in calcium handling and neurotransmitter release at the neuromuscular junction.