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Restriction fragment length polymorphism analysis using IS6110 as an epidemiological marker in tuberculosis
I Otal1, C Martín, V Vincent-Lévy-Frebault
1Unité Génie Microbiologique, Centre National de la Recherche Scientifique, Paris, France.
Journal of Clinical Microbiology
|June 1, 1991
Summary
The insertion sequence IS6110, found in multiple copies in Mycobacterium tuberculosis, remains stable in patients over 2-3 years. This confirms IS6110 as a reliable marker for tuberculosis (TB) epidemiology.
Area of Science:
- Microbiology
- Genetics
- Epidemiology
Background:
- The insertion sequence IS6110 is present in multiple copies in the Mycobacterium tuberculosis genome.
- IS6110 is a key element for the molecular typing of M. tuberculosis strains.
Purpose of the Study:
- To evaluate the genomic stability of IS6110 in Mycobacterium tuberculosis over time.
- To confirm the utility of IS6110 as an epidemiological marker for tuberculosis.
Main Methods:
- IS6110 restriction fragment length polymorphism (RFLP) analysis was performed.
- Strains were isolated from tuberculosis patients over a 2- to 3-year period.
Main Results:
- Identical IS6110 RFLP patterns were observed in strains from the same patient over 2-3 years.
- High polymorphism was detected between strains from different patients.
- IS6110 did not induce major in vivo genomic rearrangements within this timeframe.
Conclusions:
- The IS6110 insertion sequence is genetically stable in vivo over 2- to 3-year periods.
- IS6110 RFLP analysis is a valuable and reliable tool for tuberculosis outbreak investigations and epidemiological studies.