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Tyramide signal amplification for DNA and mRNA in situ hybridization

Ernst J M Speel1, Anton H N Hopman, Paul Komminoth

  • 1Department of Molecular Cell Biology, Research Institute Growth and Development (GROW), University of Maastricht, Maastricht, The Netherlands.

Methods in Molecular Biology (Clifton, N.J.)
|June 20, 2006
PubMed

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In-situ Hybridization02:31

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...