Inhibition of cell proliferation by apolipoprotein E isoform expression
1Department of Biochemistry, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China.
Abstract:
The anti-atherogenic effects of human apolipoprotein E3 (apoE3) have been partially attributed to its anti-proliferation properties. We studied if endogenously expressed apoE elicits isoform-dependent effects on cell proliferation. Rat F111 fibroblasts without native expression of apoE were used to establish cell lines with stable expression of the three human apoE isoforms. Cell growth curve studies showed that expression of apoE isoforms prolonged cell population doubling time in an isoform-dependent manner with apoE3 showing the most potent effect followed by apoE2 and apoE4 exhibiting comparable effects. Interestingly, saturation density of cell population was significantly reduced by the expression of apoE4 isofom. Further analyses revealed that all three apoE isoforms significantly lengthened G0/G1 phase (p < 0.05) of the cell cycle and were associated with the suppression of ERK1/2 activities. However, these changes were not sufficient to explain the isoform-dependent effects of apoE expression on cell population doubling time and saturation density.
More Related Videos
Related Concept Videos
Abnormal Proliferation
Inhibition of Cdk Activity
Inhibition of CDK Activity
Negative Regulator Molecules
Cell Specific Gene Expression
PI3K/mTOR/AKT Signaling Pathway

