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Enhancement of soluble protein expression through the use of fusion tags

Dominic Esposito1, Deb K Chatterjee

  • 1Protein Expression Laboratory, Research Technology Program, SAIC-Frederick, Inc, NCI-Frederick, Frederick, MD 21702, USA.

Current Opinion in Biotechnology
|June 20, 2006
PubMed

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Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...