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Updated: Aug 7, 2026

Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach
Published on: December 4, 2021
Fast and high-resolution ion chromatography at high pH on short columns packed with 1.8 microm surfactant coated
Sarah Pelletier1, Charles A Lucy
1Department of Chemistry, Gunning/Lemieux Chemistry Centre, University of Alberta, Edmonton, Alta. T6G 2G2, Canada.
Abstract:
Rapid ion chromatographic separations of small inorganic anions are performed on columns packed with high-pH resistant Zorbax Extend-C18 1.8 microm silica particles. Seven anions (iodate, chloride, nitrite, bromide, nitrate, phosphate, sulphate) are separated with 1.3 and 2 cm long x 0.46 cm I.D. C18 columns coated with the surfactant didodecyldimethylammonium bromide (DDAB). A 40 s separation is achieved at 2 mL/min with a 2.5 mM 4-hydroxybenzoic acid eluent at pH 10. Finally, the DDAB removal procedure is improved to eliminate the pressure build-up caused by precipitation of the surfactant in the column upon uncoating.
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