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Related Experiment Videos

Dry beveling micropipettes using a computer hard drive.

James G Canfield1

  • 1Department of Psychology, University of Washington, Seattle, WA 98195, USA. jamescan@u.washington.edu

Journal of Neuroscience Methods
|June 20, 2006
PubMed
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This study presents a simple, inexpensive method for dry beveling glass micropipettes using a computer hard drive. Beveled pipettes produced superior intracellular recordings from cichlid Mauthner neurons compared to sharp or broken tips.

Area of Science:

  • Neuroscience
  • Electrophysiology
  • Biomedical Engineering

Background:

  • Optimizing micropipette tip profiles is crucial for effective electrophysiological recordings.
  • Commercial beveling machines are expensive, posing a barrier to testing beveled pipette efficacy.
  • Alternative methods like pipette pullers and tip bumping exist but may not be ideal.

Purpose of the Study:

  • To develop and present a cost-effective method for dry beveling glass micropipettes.
  • To evaluate the performance of dry-beveled pipettes for intracellular recordings.
  • To provide preliminary data supporting the justification for purchasing commercial beveling equipment.

Main Methods:

  • Utilized a surplus computer hard drive to create a system for reproducible dry beveling of glass micropipettes.

Related Experiment Videos

  • Compared the performance of dry-beveled pipettes against sharp and broken tip electrodes.
  • Conducted intracellular recordings from cichlid Mauthner neurons.

    • Main Results:

    • Pipettes dry-beveled using the computer hard drive system demonstrated superior performance.
    • Dry-beveled pipettes yielded better intracellular recordings from cichlid Mauthner neurons compared to other tip types.
    • The inexpensive apparatus provided preliminary data on beveling efficacy.

    Conclusions:

    • A simple, inexpensive dry beveling system using a computer hard drive is effective for preparing superior micropipettes.
    • This method offers a viable alternative for researchers considering the purchase of commercial beveling machines.
    • The findings support the use of dry-beveled pipettes for enhanced intracellular recordings in specific neuronal preparations.