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Induction of interleukin-6 release from monocytes by serine proteinases and its potential mechanisms

T Li1, H Wang, S He

  • 1Allergy and Inflammation Research Institute, The Key Immunopharmacology Laboratory of Guangdong Province, Shantou University Medical College, Shantou 515031, Guangdong Province, China.

Scandinavian Journal of Immunology
|June 21, 2006
PubMed

Insights

Serine proteinases, including thrombin and trypsin, significantly increase interleukin-6 (IL-6) secretion from monocytes. This effect is mediated through proteinase-activated receptors (PARs), highlighting a key pathway in inflammatory responses.

Area of Science:

  • Immunology
  • Biochemistry

Background:

  • Serine proteinases are implicated in inflammation via proteinase-activated receptors (PARs).
  • The specific role of serine proteinases and PARs in interleukin-6 (IL-6) secretion from monocytes remains unclear.

Purpose of the Study:

  • To investigate the influence of serine proteinases and PAR activation on IL-6, IL-1beta, and IL-12 secretion from human monocytes.

Main Methods:

  • Highly purified human peripheral blood monocytes were challenged with various serine proteinases and PAR-activating peptides.
  • Interleukin levels in culture supernatants were quantified using enzyme-linked immunosorbent assay (ELISA).

Main Results:

  • Thrombin, trypsin, tryptase, and elastase significantly increased IL-6 release (up to 2.9-fold) from monocytes.
  • PAR-1 and PAR-4 activating peptides, but not PAR-3, also stimulated IL-6 secretion.
  • Neither serine proteinases nor PAR agonists affected IL-1beta or IL-12 secretion.

Conclusions:

  • Serine proteinases induce IL-6 secretion from monocytes, likely through the activation of PAR-1 and PAR-4.
  • This pathway represents a novel mechanism in monocyte inflammatory signaling, specifically for IL-6 production.

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