Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A continuous assay for DNA cleavage using molecular break lights.

John B Biggins1, James R Prudent, David J Marshall

  • 1Laboratory for Biosynthetic Chemistry, Pharmaceutical Sciences Division, School of Pharmacy, University of Wisconsin, Madison, WI, USA.

Methods in Molecular Biology (Clifton, N.J.)
|June 21, 2006
PubMed
Summary

Researchers developed a new "molecular break light assay" to detect DNA cleavage. This sensitive and rapid method uses a modified oligonucleotide and is adaptable for high-throughput screening of DNA-cleaving agents.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tetracenomycin Aglycones Primarily Inhibit Cell Growth and Proliferation in Mammalian Cancer Cell Lines.

Applied sciences (Basel, Switzerland)·2026
Same author

The Utility of Salivary 11-Dehydrodexamethasone as a Marker of Dexamethasone Absorption.

The Journal of clinical endocrinology and metabolism·2026
Same author

Bridging Tumor Growth Dynamics and Survival in Preclinical Oncology Drug Development - A Translational Tumor Growth Inhibition and Time to Event (TGI-TTE) Modeling Framework.

Research square·2026
Same author

Physiologically Based Pharmacokinetic Modeling to Predict Human Pharmacokinetics of a Novel Mithramycin Analog for Ewing Sarcoma.

Research square·2026
Same author

Correction to: Surgical screening protocol for craniocervical instability secondary to ehlers-danlos syndrome and other connective tissue disorders: analysis of a 347 patient case series.

Neurosurgical review·2026
Same author

Nonlinear Mixed-Effects Modeling to Characterize the Pharmacokinetics of a Novel Mithramycin Analogue for Ewing Sarcoma in Mice.

Research square·2026

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Analytical Chemistry

Background:

  • Investigating DNA-cleaving molecules like nucleases and antibiotics offers laboratory and clinical benefits.
  • A simple, continuous assay for detecting DNA cleavage has been a significant unmet need.

Purpose of the Study:

  • To introduce a novel, sensitive, and rapid assay for detecting DNA cleavage.
  • To provide a convenient method adaptable for high-throughput screening of DNA-cleaving agents.

Main Methods:

  • A "molecular break light assay" was developed using a single oligonucleotide.
  • The oligonucleotide features a 5'-fluorophore-3'-quencher pair in a stem-loop structure.
  • The assay incorporates a specific DNA recognition site for cleavage detection.

Related Experiment Videos

Main Results:

  • The assay enables rapid and highly sensitive detection of DNA cleavage.
  • The methodology is easily adaptable to standard laboratory fluorometers and multi-well plate systems.
  • This facilitates high-throughput screening of novel DNA-cleaving agents.

Conclusions:

  • The molecular break light assay addresses the need for a simple and continuous DNA cleavage detection method.
  • The assay's adaptability supports high-throughput screening for new DNA-cleaving molecules.
  • Potential applications include transcription factor protection assays.