Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Multiparameter immunofluorescence on paraffin-embedded tissue sections.

Frauke Bataille1, Sabine Troppmann, Frank Klebl

  • 1Institute of Pathology, University of Regensburg, Germany. frauke.bataille@klinik.uni-regensburg.de

Applied Immunohistochemistry & Molecular Morphology : AIMM
|June 21, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The canine vaginal microbiome during heat and fertility in healthy breeding dogs.

PloS one·2025
Same author

Integrating the Patient Perspective to Validate a Measure of Disease Severity in Inflammatory Bowel Disease: Online Survey of Patients and Their Physicians.

Inflammatory bowel diseases·2024
Same author

The Role of T-Cadherin (CDH13) in Treatment Options with Garcinol in Melanoma.

Cancers·2024
Same author

Transcription factor activating enhancer-binding protein 2ε (AP2ε) modulates phenotypic plasticity and progression of malignant melanoma.

Cell death & disease·2024
Same author

Loss of miR-101-3p in melanoma stabilizes genomic integrity, leading to cell death prevention.

Cellular & molecular biology letters·2024
Same author

Alternative Wnt-signaling axis leads to a break of oncogene-induced senescence.

Cell death & disease·2024
Same journal

ISIMM Page.

Applied immunohistochemistry & molecular morphology : AIMM·2026
Same journal

Clinicopathologic Features of Warthin-Like Subtype of Papillary Thyroid Carcinoma.

Applied immunohistochemistry & molecular morphology : AIMM·2026
Same journal

HER2 In Situ Hybridization: Validation and Implementation of Brightfield Assay in a US Academic Pathology Laboratory.

Applied immunohistochemistry & molecular morphology : AIMM·2026
Same journal

Keratoameloblastoma of the Jaw Bones: Description of 2 Rare Cases and Cytokeratin Profile.

Applied immunohistochemistry & molecular morphology : AIMM·2026
Same journal

Role of Twist and Vimentin as Epithelial-Mesenchymal Transition Biomarkers in Oral Squamous Cell Carcinoma: Relation to Cancer-Associated Fibroblasts.

Applied immunohistochemistry & molecular morphology : AIMM·2026
Same journal

Exploring the Immune Microenvironment for Predicting Immunotherapy Efficacy in Epstein-Barr Virus-Associated Gastric Cancer.

Applied immunohistochemistry & molecular morphology : AIMM·2026
See all related articles

This study presents a standardized immunofluorescence technique for multicolor labeling of three and four primary antibodies in paraffin-embedded tissues. The method enhances antigen detection in diagnostics and research by overcoming limitations of previous multi-labeling approaches.

Area of Science:

  • Histopathology
  • Immunofluorescence
  • Biomedical Research

Background:

  • Immunohistochemical techniques are vital in diagnostics and research.
  • Formalin-fixed, paraffin-embedded tissues offer excellent morphology but have limitations for multi-antigen detection via immunofluorescence.
  • Previous triple labeling studies showed inferior signal intensity compared to double labeling.

Purpose of the Study:

  • To develop and standardize a multicolor immunofluorescence technique for detecting multiple primary antibodies in paraffin-embedded tissue sections.
  • To improve signal intensity and reduce background staining in triple and quadruple labeling.
  • To overcome limitations of existing immunofluorescence methods for simultaneous antibody detection.

Main Methods:

  • Standardized immunofluorescence technique applied to routinely processed paraffin-embedded human tissue sections.

Related Experiment Videos

  • Demonstration of multicolor labeling with three and four primary antibodies.
  • Development of procedures to minimize background and nonspecific double staining.

    • Main Results:

    • Successful multicolor labeling of three and four primary antibodies was achieved in paraffin-embedded tissue sections.
    • The standardized technique demonstrated robust signal intensity for multiple antigens.
    • Effective methods for reducing background and nonspecific staining were implemented.

    Conclusions:

    • The presented immunofluorescence technique enables reliable multicolor labeling of multiple antibodies in paraffin-embedded tissues.
    • This advancement improves antigen detection capabilities for complex diagnostic and research applications.
    • The standardized protocol offers a valuable tool for enhanced histopathological analysis.