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Related Experiment Videos

Development of a self-proliferating Leydig cell line: a hyper-sensitive E-screening model.

James W DuMond1, Kamaleshwar P Singh, Deodutta Roy

  • 1Department of Biology, Texas Southern University, 210 Nabrit Science Building, Houston, TX 77004, USA. dumond_jw@tsu.edu

Oncology Reports
|June 21, 2006
PubMed
Summary

A new mouse Leydig cell line (TM3-SF) was developed to study estrogenic endocrine disruption. This cell line shows estrogen sensitivity and proliferation, with inhibin/activin involved in its growth regulation.

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Area of Science:

  • Endocrinology
  • Reproductive Biology
  • Toxicology

Background:

  • Mechanisms of estrogenic endocrine disruption on the male reproductive tract are not well understood.
  • Xenobiotic chemicals can possess estrogenic properties affecting male reproductive tissues.
  • A novel cell line is needed to investigate these effects.

Purpose of the Study:

  • To develop a self-proliferating mouse Leydig cell line (TM3-SF) for studying estrogenic effects.
  • To assess the sensitivity of TM3-SF cells to known estrogenic compounds.
  • To investigate the underlying mechanisms of TM3-SF cell proliferation.

Main Methods:

  • Development of TM3-SF cell line from TM3 cells through progressive serum starvation.
  • Maintenance of TM3-SF cells under serum-free conditions for 20 passages.

Related Experiment Videos

  • Assaying estrogen sensitivity via cell proliferation studies using diethylstilbestrol (DES), 17beta-estradiol, 17alpha-estradiol, and Bis-phenol A.
  • Investigating growth mechanisms using the anti-betaa subunit of inhibin/activin.

    • Main Results:

    • TM3-SF cells demonstrated significant proliferation in response to all tested estrogenic compounds (DES, Bis-phenol A, 17beta-estradiol, 17alpha-estradiol) at concentrations from 1 pg/ml to 100 ng/ml.
    • The observed order of potency was DES > Bis-phenol A > 17beta-estradiol and > 17alpha-estradiol.
    • Inhibin or activin was identified as a primary growth factor, with the anti-betaa subunit inhibiting growth by 50% and blocking DES-induced proliferation.

    Conclusions:

    • The TM3-SF cell line is a valuable tool for studying estrogenic endocrine disruption in male reproductive tissues.
    • TM3-SF cell growth is estrogen-sensitive.
    • Inhibin or activin plays a crucial role in the self-regulation of TM3-SF cell growth.