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Distribution of Cytoplasmic Content

Distribution of Cytoplasmic Content

Cytokinesis segregates a cell’s chromosomes and organelles into its daughter cells. Organelles divide and grow prior to cell division but cannot be synthesized de novo; therefore, cells must receive at least one copy of each organelle to survive. Currently, many of the details of how the organelles are distributed are not yet fully elucidated.
Distribution of cytoplasmic determinants
The cytoplasm contains various organelles, as well as salts, proteins, and water. The distribution of small...

Extraction: Partition and Distribution Coefficients

Extraction: Partition and Distribution Coefficients

The distribution law or Nernst's distribution law is the law that governs the distribution of a solute between two immiscible solvents. This law, also known as the partition law, states that if a solute is added to the mixture of two immiscible solvents at a constant temperature, the solute is distributed between the two solvents in such a way that the ratio of solute concentrations in the solvents remains constant at equilibrium.
For extracting a solute from an aqueous phase into an organic...

Distribution of Cytoplasmic Content

Distribution of Cytoplasmic Content

Cytokinesis segregates a cell’s chromosomes and organelles into its daughter cells. Organelles divide and grow prior to cell division but cannot be synthesized de novo; therefore, cells must receive at least one copy of each organelle to survive. Currently, many of the details of how the organelles are distributed are not yet fully elucidated.
Distribution of cytoplasmic determinants
The cytoplasm contains various organelles, as well as salts, proteins, and water. The distribution of small...

Moisture Content and Bulking of Aggregate

Moisture Content and Bulking of Aggregate

The moisture content of aggregates is a crucial factor in construction, particularly in concrete mixing, as it influences the total water required in the mix. Moisture content represents the water coated on the exterior surface of the aggregate existing in a saturated and surface-dry condition. The total water content of a moist aggregate is the sum of its moisture content and water absorption.
When aggregates are exposed to rain or sit in stockpiles, they absorb moisture, which must be...

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Related Experiment Video

Updated: Jun 25, 2026

Method to Produce Durable Pellets at Lower Energy Consumption Using High Moisture Corn Stover and a Corn Starch Binder in a Flat Die Pellet Mill

Method to Produce Durable Pellets at Lower Energy Consumption Using High Moisture Corn Stover and a Corn Starch Binder in a Flat Die Pellet Mill

Published on: June 15, 2016

Protein distribution in commercial wet- and dry-milled corn germ.

Nicholas Parris¹, Robert A Moreau, David B Johnston

¹Eastern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, 600 East Mermaid Lane, Wyndmoor, Pennsylvania 19038, USA. nparris@errc.ars.usda.gov

Journal of Agricultural and Food Chemistry

|June 22, 2006

Summary

This summary is machine-generated.

Processing corn germ significantly impacts protein extraction. Dry-milling preserves more corn germ proteins compared to wet-milling, yielding higher-value coproducts.

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Quantifying Plant Soluble Protein and Digestible Carbohydrate Content, Using Corn (Zea mays) As an Exemplar

Quantifying Plant Soluble Protein and Digestible Carbohydrate Content, Using Corn (Zea mays) As an Exemplar

Published on: August 6, 2018

Area of Science:

Agricultural Science
Biochemistry
Food Science

Background:

Corn germ is a valuable byproduct of corn processing.
Understanding corn germ protein behavior is crucial for identifying high-value coproducts.
Processing methods can significantly alter protein characteristics.

Purpose of the Study:

To determine the effect of commercial processing conditions on corn germ proteins.
To identify high-valued coproducts from commercially processed corn germ.

Main Methods:

Extraction of corn germ proteins using Tris, sodium dodecyl sulfate (SDS) buffer with 2-mercaptoethanol.
Analysis of protein profiles using SDS-polyacrylamide gel electrophoresis (SDS-PAGE).
Comparison of protein content and nonprotein nitrogen values between wet- and dry-milled corn germ.

Main Results:

Significantly less protein was extracted from wet-milled compared to dry-milled corn germ.
SDS-PAGE revealed diverse protein molecular masses (10–66 kDa) in dry-milled germ versus limited bands (~23 kDa) in wet-milled germ.
Wet-milled corn germ had higher nonprotein nitrogen values, indicating potential protein degradation.

Conclusions:

Commercial wet-milling processing significantly reduces the extractable protein yield from corn germ compared to dry-milling.
Protein profiles differ substantially between wet- and dry-milled corn germ.
Laboratory preparations of wet-milled corn germ more closely resemble dry-milled germ, suggesting microbial activity during commercial wet-milling steeping impacts protein integrity.