Sleeping Beauty transposon system--future trend in T-cell-based gene therapies?
Ruth Frommolt1, Florian Rohrbach, Matthias Theobald
1Department of Hematology & Oncology, Johannes Gutenberg University of Mainz, Langenbeckstr. 1, 55101 Mainz, Germany. frommolt@uni-mainz.de
Abstract:
The Sleeping Beauty (SB) transposon system can mediate stable gene transfer and expression in primary human T cells. Optimal in vitro conditions for maximum gene transfer efficiencies have been developed with regard to further application of the SB transposon system in T cell based gene therapies. This raises the question of whether or not the SB transposon system is a convincing alternative for virus-mediated gene transfer based on the currently available data. Here, we will discuss controversial safety and efficacy issues in transposon and viral gene transfer technology.
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