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Related Experiment Videos

The intrinsic coagulation activity assay.

Thomas W Stief1, Stefanie Otto, Harald Renz

  • 1Department of Clinical Chemistry - Central Laboratory, University Hospital Giessen & Marburg, Germany. thstief@med.uni-marburg.de

Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis
|June 22, 2006
PubMed
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A novel intrinsic coagulation activity assay (INCA) quantifies thrombin generation, reflecting normal hemostasis and enabling low-molecular-weight heparin monitoring. This assay provides results as a percentage of normal thrombin activity.

Area of Science:

  • Coagulation and Hemostasis Research
  • Biochemical Assays and Diagnostics
  • Thrombin Generation Measurement

Background:

  • Assays for contact-phase-mediated thrombin generation are crucial for understanding hemostasis.
  • Existing methods may alter plasma matrix or provide results in coagulation seconds, limiting interpretation.
  • A need exists for a sensitive assay reflecting normal intrinsic hemostasis and suitable for monitoring therapeutics.

Purpose of the Study:

  • To develop and validate a new assay for measuring contact-phase-mediated thrombin generation.
  • To establish the intrinsic coagulation activity assay (INCA) for quantifying thrombin activity.
  • To assess the utility of INCA for reflecting normal hemostasis and monitoring low-molecular-weight heparins.

Main Methods:

Related Experiment Videos

  • Developed the intrinsic coagulation activity assay (INCA) using citrated plasma, SiO2, and CaCl2.
  • Incubated samples for specific reaction times (4 and 5 minutes) at 37°C.
  • Inhibited activation with arginine, added a chromogenic thrombin substrate, and measured absorbance at 405 nm.

    • Main Results:

    • The INCA assay successfully measures thrombin generation, with normal ranges established for INCA-4 and INCA-5.
    • Assay results are presented as a percentage of normal generated thrombin activity, not coagulation seconds.
    • The INCA assay demonstrated its ability to reflect normal intrinsic hemostasis and monitor low-molecular-weight heparins.

    Conclusions:

    • The intrinsic coagulation activity assay (INCA) is a reliable method for quantifying thrombin generation.
    • INCA provides a clinically relevant measure of intrinsic hemostasis.
    • This assay offers advantages over traditional methods by maintaining plasma integrity and providing percentage-based results.