Multicell tumor spheroids in photodynamic therapy

Area of Science:

• Oncology
• Biomedical Engineering
• Photomedicine

Background:

• Multicell spheroids (MCSs) offer a simplified in vitro system for evaluating investigational treatments.
• MCSs have been instrumental in understanding photodynamic therapy (PDT) parameters, including sensitizer photobleaching.
• MCSs facilitate the study of complex PDT regimens, such as multiple treatments and combined therapies with radiation or hyperthermia.

Purpose of the Study:

• To review the current literature on MCS applications in PDT.
• To present novel MCS models for investigating tumor cell invasion and angiogenesis in high-grade gliomas.
• To explore the utility of in silico models for predicting PDT treatment responses.

Main Methods:

• Literature review of MCS studies in PDT.
• Development of in vivo brain tumor models using human tumor spheroids in immunodeficient rats.
• Utilizing computer simulations (in silico models) to describe MCS growth and predict therapeutic effects.

Main Results:

• Elucidation of PDT sensitizer photobleaching mechanisms through MCS studies.
• Progress in developing an in vivo brain tumor model with biopsy-derived human tumor spheroids.
• In silico models have generated verifiable predictions for MCS growth and PDT responses.

Conclusions:

• MCSs are versatile tools for PDT research, aiding in understanding mechanisms and treatment strategies.
• Advanced in vivo and in silico models are emerging for more accurate brain tumor and PDT investigations.
• Computer simulations show promise in guiding the development of improved PDT therapeutics.