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Density differences between hybrid and nonhybrid particles in two adenovirus-simian virus 40 hybrid populations
S G Baum1, W H Wiese, W P Rowe
1Departments of Medicine and Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461.
Abstract:
The adenovirus 7-simian virus 40 hybrid virus population E46(+) was subjected to fixed-angle equilibrium density gradient centrifugation in CsCl. A difference in buoyant density between the hybrid virion and its nonhybrid adenovirus 7 counterpart was noted, the hybrid virion possessing the lower buoyant density. This difference in buoyant density appeared to be accentuated in a population of adenovirus 2(+t7), a derivative of E46(+) in which the adenovirus 7-simian virus 40 genome had been transferred to an adenovirus 2 capsid.
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