Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Laurdan in fluid bilayers: position and structural sensitivity.

Cíntia C De Vequi-Suplicy1, Carlos R Benatti, M Teresa Lamy

  • 1Instituto de Física, Universidade de S. Paulo, CP 66318, CEP 05315-970, Sao Paulo, SP, Brazil.

Journal of Fluorescence
|June 23, 2006
PubMed
Summary

Laurdan, a fluorescent probe, is sensitive to lipid packing in membranes, even in fluid states. This sensitivity is crucial for understanding membrane dynamics and probe behavior.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Physicochemical Characterization of Anionic Lipid Membranes under Low and Physiological Ionic Strength: Effects of Moxifloxacin Assessed by Calorimetry, Spin-Label, Steady-State, and Time-Resolved Fluorescence.

ACS omega·2026
Same author

Corrigendum to "Effect of disialoganglioside GD3 on the subgel, gel and fluid phases of cationic DODAB vesicles" [biophysical chemistry 326 (2025) 107503].

Biophysical chemistry·2025
Same author

Effect of disialoganglioside GD3 on the subgel, gel and fluid phases of cationic DODAB vesicles.

Biophysical chemistry·2025
Same author

DODAB-ODN lipoplex structure is highly dependent on ODN concentration: A multitechnique experimental study.

Biochemistry and biophysics reports·2025
Same author

New insights into the interaction of emodin with lipid membranes.

Biophysical chemistry·2024
Same author

Evidence for Ca<sup>2+</sup>-induced structural change in diluted GD3 ganglioside dispersions.

Biochimica et biophysica acta. Biomembranes·2024

Area of Science:

  • Biophysics
  • Membrane Biophysics
  • Fluorescence Spectroscopy

Background:

  • Laurdan (2-dimethylamino-6-lauroylnaphthalene) is a hydrophobic fluorescent probe extensively used in lipid systems.
  • Its fluorescence is sensitive to lipid phases, microenvironment polarity, and viscosity.

Purpose of the Study:

  • To investigate the temperature dependence of Laurdan fluorescence in different lipid systems.
  • To determine Laurdan's sensitivity to lipid bilayer packing, particularly in fluid membranes.

Main Methods:

  • Incorporation of Laurdan into 1,2-dipalmitoyl-sn-glycero-3-[phospho-rac-(1-glycerol)] (DPPG) and 1,2-dilauroyl-sn-glycero-3-phosphocholine (DLPC) lipid bilayers.
  • Analysis of Laurdan fluorescent emission via decomposition into Gaussian bands.
  • Utilizing Laurdan fluorescence anisotropy and spin labels to probe bilayer packing and quenching.

Related Experiment Videos

Main Results:

  • Laurdan fluorescence strongly correlates with the gel-fluid phase transition of DPPG.
  • Laurdan fluorescence in DLPC (fluid phase) is temperature-dependent, indicating sensitivity to bilayer packing.
  • Results from anisotropy and spin labeling confirm Laurdan's sensitivity to membrane packing and suggest a shallow membrane insertion depth.

Conclusions:

  • Laurdan fluorescence is highly sensitive to lipid bilayer packing, extending to fluid membranes.
  • This sensitivity provides valuable insights into membrane dynamics and the behavior of hydrophobic probes.
  • Laurdan's shallow membrane position influences its fluorescence response to lipid packing.