Related Experiment Video
Updated: Jul 9, 2026

Analyzing the Function of Small GTPases by Microinjection of Plasmids into Polarized Epithelial Cells
Published on: May 31, 2011
Assignment of G-protein subtypes to specific receptors inducing inhibition of calcium currents
C Kleuss1, J Hescheler, C Ewel
1Institut für Molekularbiologie und Biochemie, Freie Universität Berlin, Germany.
Abstract:
The inhibition of voltage-dependent Ca2+ channels in secretory cells by plasma membrane receptors is mediated by pertussis toxin-sensitive G proteins. Multiple forms of G proteins have been described, differing principally in their alpha subunits, but it has not been possible to establish which G-protein subtype mediates inhibition by a specific receptor. By intranuclear injection of antisense oligonucleotides into rat pituitary GH3 cells, the essential role of the Go-type G proteins in Ca(2+)-channel inhibition is established: the subtypes Go1 and Go2 mediate inhibition through the muscarinic and somatostatin receptors, respectively.
Related Concept Videos
GTPases and their Regulation
Large G-proteins, also known...
Position-effect Variegation
Eukaryotic Transcription Inhibitors
Eukaryotic transcription inhibitors usually contain two distinct domains, a DNA...
Activation and Inactivation of G Proteins
GPCR Desensitization
G-Protein Gated Ion Channels
Sensory organs,...

