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Updated: Aug 7, 2026
Lymphocyte Isolation from Human Skin for Phenotypic Analysis and Ex Vivo Cell Culture
Published on: April 8, 2016
Age-associated decrease of CD1d protein production in normal human skin
M A Adly1, H A Assaf, M R Hussein
1Department of Zoology, Sohag Faculty of Science, South Valley University, Sohag, Egypt, and Department of Dermatology and Venereology, Faculty of Medicine, University Hospital of Hamburg-Eppendorf, Germany.
Background:
CD1d belongs to a family of antigen-presenting molecules structurally related to the classical major histocompatibility complex class I proteins.
Objectives:
To examine the expression pattern of CD1d protein in normal human skin with ageing.
Methods:
Twenty normal human skin biopsy specimens were obtained from 20 healthy individuals. The latter were divided into three age groups: children (5-20 years), adults (21-50 years) and the elderly (51-81 years). The intensity of CD1d protein production was examined in human skin using immunofluorescent and immunoalkalinephosphatase staining methods.
Results:
In the epidermis, CD1d protein production was strong in the skin of the children and declined gradually with age, being moderate in adults and weak in the elderly. As compared with values in children, there was a statistically significant decrease (P<0.05) in CD1d protein production in the elderly. In the dermis, CD1d protein production was strong in the fibroblasts, sweat glands, sebaceous glands, blood vessels and hair follicles regardless of age.
Conclusions:
Our study reports a decreased CD1d protein production in normal human skin with ageing. The clinical ramifications of these observations mandate further investigations.
