Related Experiment Videos
The angiosuppressive effects of 20(R)- ginsenoside Rg3
Patrick Y K Yue1, Daisy Y L Wong, P K Wu
1Hung Lai Ching Laboratory of Biomedical Science, Research and Development Division, School of Chinese Medicine, Hong Kong Baptist University, Hong Kong.
Biochemical Pharmacology
|June 24, 2006
Summary
20(R)-ginsenoside Rg3 (Rg3), a compound from ginseng, effectively inhibits tumor angiogenesis. Rg3 suppressed endothelial cell proliferation, migration, and new blood vessel formation, demonstrating its anti-tumor potential.
Area of Science:
- Oncology
- Pharmacology
- Cell Biology
Background:
- Aberrant angiogenesis is crucial for solid tumor progression.
- Anti-angiogenic therapy offers a promising strategy for tumor growth control.
Purpose of the Study:
- To investigate the anti-angiogenic potential of 20(R)-ginsenoside Rg3 (Rg3).
- To evaluate Rg3's effects on various steps of angiogenesis.
Main Methods:
- In vitro assays: human umbilical vein endothelial cell (HUVEC) proliferation, capillary tube formation, and chemoinvasion.
- Ex vivo assays: rat aortic ring assay for microvascular sprouting.
- In vivo assay: Matrigel plug assay for angiogenesis.
- Analysis of Matrix metalloproteinases (MMPs) activity.
Main Results:
- Rg3 inhibited HUVEC proliferation (IC50 = 10 nM) and capillary tube formation.
- Rg3 attenuated VEGF-induced HUVEC chemoinvasion and microvascular sprouting.
- Rg3 suppressed bFGF-induced angiogenesis in vivo and reduced MMP-2/MMP-9 activity.
Conclusions:
- Rg3 exhibits significant angiosuppressive activity.
- Rg3 demonstrates anti-tumor properties by inhibiting angiogenesis.