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Related Experiment Videos

Functional assays for replication protein A (RPA).

Sara K Binz1, Anne M Dickson, Stuart J Haring

  • 1Department of Biochemistry, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, USA.

Methods in Enzymology
|June 24, 2006
PubMed
Summary
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Replication protein A (RPA), crucial for DNA replication and repair, is analyzed using various methods. This chapter details techniques for studying RPA interactions, functions, and cellular roles.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • Replication protein A (RPA) is a vital heterotrimeric, single-stranded DNA-binding protein conserved across eukaryotes.
  • RPA plays essential roles in DNA replication, repair, recombination, and coordinating cellular responses to DNA damage.

Purpose of the Study:

  • To provide an overview of methods for analyzing RPA.
  • To detail techniques for assessing RPA-DNA and RPA-protein interactions.
  • To discuss methods for evaluating RPA's functional activities and cellular effects.

Main Methods:

  • Analysis of RPA-DNA interactions.
  • Analysis of RPA-protein interactions.
  • Functional assays for RPA activities.
  • Cellular visualization of RPA.

Related Experiment Videos

  • Assessment of RPA's impact on cell cycle progression.

    • Main Results:

    • Established assays allow for the comprehensive study of RPA.
    • Methods cover diverse aspects of RPA biology, from molecular interactions to cellular functions.
    • The chapter outlines techniques applicable to mammalian cells.

    Conclusions:

    • A range of methods exist for thoroughly investigating Replication protein A.
    • These techniques are crucial for understanding RPA's multifaceted roles in DNA metabolism and cellular health.
    • The described methods facilitate research into DNA replication, repair, and damage response pathways.