Related Experiment Video
Updated: Aug 7, 2026

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage
Published on: August 21, 2021
Methods for studying the cellular response to DNA damage: influence of the Mre11 complex on chromosome metabolism
Jan-Willem F Theunissen1, John H J Petrini
1Memorial Sloan Kettering Cancer Center, New York, New York, USA.
Abstract:
Dramatic progress in understanding the mediators and mechanisms of chromosome break metabolism has been made in recent years. As a result, the links between disease and defects in chromosome dynamics have become clearer. In this chapter, we discuss techniques employed in our laboratory to study chromosome break metabolism, which include assessments at the molecular and cellular level. In our laboratory, we use the Mre11 complex as a tool to study this process, but the techniques discussed are of general relevance.
Related Concept Videos
Nucleotide Excision Repair
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
Nucleotide Excision Repair
DNA Damage Can Stall the Cell Cycle
DNA Damage can Stall the Cell Cycle
Homologous Recombination
Overview of DNA Repair
Chemically...
