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Related Experiment Videos

Structure-function analysis of SWI2/SNF2 enzymes.

Harald Dürr1, Karl-Peter Hopfner

  • 1Department of Chemistry and Biochemistry, Gene Center, Ludwig Maximilians University of Munich, Munich, Germany.

Methods in Enzymology
|June 24, 2006
PubMed
Summary
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SWI2/SNF2 enzymes are molecular motors that use ATP to move along DNA, disrupting protein:DNA complexes. This chapter details methods for studying their DNA translocation and distortion mechanisms.

Area of Science:

  • Biochemistry
  • Structural Biology
  • Molecular Biology

Background:

  • SWI2/SNF2 enzymes are ATP-dependent molecular motors involved in chromatin remodeling and DNA repair.
  • These enzymes utilize ATP hydrolysis to translocate duplex DNA through a helicase-like motor domain.

Purpose of the Study:

  • To describe biochemical and structural methods for investigating the molecular mechanisms of SWI2/SNF2 enzymes.
  • To detail assays for monitoring DNA-dependent ATPase activity, DNA translocation, and DNA distortion.
  • To present recent advances in crystallizing and determining the structures of SWI2/SNF2 enzymes bound to DNA.

Main Methods:

  • Assays for DNA-dependent ATPase activity.
  • In vitro assays for monitoring DNA translocation on duplex DNA.

Related Experiment Videos

  • Techniques to measure DNA distortion induced by SWI2/SNF2 enzymes.
  • X-ray crystallography for structure determination of enzyme-DNA complexes.

    • Main Results:

    • Established assays effectively monitor key enzymatic activities of SWI2/SNF2 enzymes.
    • Progress in obtaining high-resolution structures of SWI2/SNF2 enzymes in complex with duplex DNA.
    • Insights into the screw-like motion of DNA translocation and force generation.

    Conclusions:

    • Biochemical and structural studies provide crucial insights into the motor function of SWI2/SNF2 enzymes.
    • Understanding these mechanisms is vital for comprehending chromatin remodeling and DNA repair processes.
    • Advanced structural data will further elucidate the atomic details of DNA engagement and translocation.